Pengujian aktivitas antimikroba ekstrak daun kersen (Mutingia calabura L.) menggunakan pelarut nades (Natural Deep Eutectic Solvents) dengan metode disc diffusion

Authors

  • Hesti Marliza Universitas Lancang Kuning
  • Rury Trisa Utami Institut Kesehatan Mitra Bunda
  • Ayu Amelia Institut Kesehatan Mitra Bunda
  • Reri Pratiwi Institut Kesehatan Mitra Bunda

DOI:

https://doi.org/10.30862/accej.v8i1.889

Keywords:

Mutingia calabura L, NADES, S. aureus, E. coli

Abstract

Antibiotic resistance is one of the significant global health challenges, prompting the search for new antimicrobial compounds from natural sources. One promising plant is cherry leaves (Muntingia calabura L.), which are known to contain flavonoids and other bioactive compounds with antibacterial potential. This study evaluated the antimicrobial activity of cherry leaf extract obtained through Ultrasound-Assisted Extraction (UAE) using Natural Deep Eutectic Solvents (NADES) as extraction solvents. Two types of NADES were used: a mixture of choline chloride: citric acid (1:2) and choline chloride: glycerol (1:2), which offer advantages such as low toxicity, high stability, non-volatility, and environmental friendliness compared to conventional organic solvents. Antimicrobial activity was assessed using the disc diffusion method against Escherichia coli and Staphylococcus aureus. The results showed that the extract using choline chloride: citric acid as solvent produced inhibition zones of 22.5 mm against E. coli and 16.05 mm against S. aureus. In contrast, the extract using choline chloride glycerol exhibited low activity against E. coli (3.41 mm) and was inactive against S. aureus. These findings indicate that combining UAE and citric acid-based NADES is an effective and sustainable approach for extracting bioactive compounds to develop natural antibacterial agents.

References

Ahmad, I., & Prabowo, W. C. (2020). Optimasi Metode Ekstraksi Berbantu Mikrowave Dengan Pelarut Hijau (Asam Sitrat-Glukosa) Terhadap Kadar Polifenol Total Dari Daun Kadamba (Mitragyna Speciosa Korth. Havil) Menggunakan Response Surface Methodology. Majalah Farmasi Dan Farmakologi, 24(1), 11–16. Https://Doi.Org/10.20956/Mff.V24i1.9456

Alouw, G., Fatimawali, F., & Lebang, J. S. (2022). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kersen (Muntingia Calabura L.) Terhadap Bakteri Staphylococcus Aureus Dan Pseudomonas Aeruginosa Dengan Metode Difusi Sumuran. Jurnal Farmasi Medica/Pharmacy Medical Journal (Pmj), 5(1), 36. Https://Doi.Org/10.35799/Pmj.V5i1.41430

Andriani, M., Permana, G. D. M., & Widarta, I. W. R. (2019). The Effect Of Time And Temperature Extraction On Antioxidant Activity Of Starfruit Wuluh Leaf (Averrhoa Bilimbi L.) Using Ultrasonic Assisted Extraction (Uae) Method. Ilmu Dan Teknologi Pangan, 8(3), 330–340.

Arum, Y., Supartono, & Sudarmin. (2012). Isolasi Dan Uji Antimikroba Ekstrak Daun Kersen. Indonesian Journal Of Mathematics And Natural Sciences, 35(2), 157–164.

Bakhtra, D. D. J. (2018). Uji Aktivitas Fraksi Dari Ekstrak Daun Sambung Nyawa (Gynura Procumbens (Lour)Merr.) Terhadap Bakteri Shigella Dysenteriae. Jurnal Farmasi Higea, 10(1), 10–18. Http://Jurnalfarmasihigea.Org/Index.Php/Higea/Article/View/175

Buhian, W. P. C., Rubio, R. O., Valle, D. L., & Martin-Puzon, J. J. (2016). Bioactive Metabolite Profiles And Antimicrobial Activity Of Ethanolic Extracts From Muntingia Calabura L. Leaves And Stems. Asian Pacific Journal Of Tropical Biomedicine, 6(8), 682–685. Https://Doi.Org/10.1016/J.Apjtb.2016.06.006

Chaudhari, R. N., Jain, A. K., & Chatap, V. K. (2020). Phytochemical Screening, Antioxidant And Antimicrobial Potential Of Leaves Extract Of Muntingia Calabura. Journal Of Advanced Scientific Research, 11(4), 218–224.

Cheong, N. D. H., Amran, M. M., & Yusof, H. (2022). Phytochemical Investigation And Antimicrobial Activity Of Muntingia Calabura L. Against Selected Pathogens. Malaysian Journal Of Medicine And Health Sciences, 18, 301–307. Https://Doi.Org/10.47836/Mjmhs18.S15.42

Dai, Y. W.-J. (2013). Natural deep eutectic solvents as new potential media for green technology. Analytica Chimica Acta, 766, 61–68., 766, 61–68.

Datu, K. A. T., Fitriani, N., & Ahmad, I. (2019). Pengaruh Penggunaan Metode Lactic Acid-Sucrose Dengan Microwave Assisted Extraction (MAE) Terhadap Polifenol Total Dari Herba Suruhan (Peperomia Pellucida (L.) Kunth). Proceeding Of Mulawarman Pharmaceuticals Conferences, 10(October 2019), 114–117. Https://Doi.Org/10.25026/Mpc.V10i1.373

Dian, R., . F., & Budiarso, F. (2015). Uji Resistensi Bakteri Escherichia Coli Yang Diisolasi Dari Plak Gigi Terhadap Merkuri Dan Antibiotik Kloramfenikol. Jurnal E-Biomedik, 3(1). Https://Doi.Org/10.35790/Ebm.3.1.2015.6607

Lestari, Y., Ardiningsih, P., & Nurlina. (2016). Aktivitas Antibakteri Gram Positif Dan Negatif Dari Ekstrak Dan Fraksi Daun Nipah (Nypa Fruticans Wurmb.) Asal Pesisir Sungai Kakap Kalimantan Barat. Jurnal Kimia Khatulistiwa, 5(4), 1–8.

Magvirah, T., Marwati, & Ardhani, F. (2019). Uji Daya Hambat Bakteri Staphylococcus Aureus Menggunakan Ekstrak Daun Tahongai (Kleinhovia Hospita L.). Jurnal Peternakan Lingkungan Tropis, 2(2), 41–50.

Murray, C. J., Ikuta, K. S., Sharara, F., Swetschinski, L., Robles Aguilar, G., Gray, A., Han, C., Bisignano, C., Rao, P., Wool, E., Johnson, S. C., Browne, A. J., Chipeta, M. G., Fell, F., Hackett, S., Haines-Woodhouse, G., Kashef Hamadani, B. H., Kumaran, E. A. P., Mcmanigal, B., … Naghavi, M. (2022). Global Burden Of Bacterial Antimicrobial Resistance In 2019: A Systematic Analysis. The Lancet, 399(10325), 629–655. Https://Doi.Org/10.1016/S0140-6736(21)02724-0

Nurhamidin, A. P. R., Fatimawali, F., & Antasionasti, I. (2021). Uji Aktivitas Antibakteri Ekstrak N-Heksan Biji Buah Langsat (Lansium Domesticum Corr) Terhadap Bakteri Staphylococus Aureus Dan Klebsiella Pneumoniae. Pharmacon, 10(1), 748. Https://Doi.Org/10.35799/Pha.10.2021.32772

Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran Dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41. Https://Doi.Org/10.24198/Jthp.V1i2.27537

Paat, E. M., Wewengkang, D. S., & Rotinsulu, H. (2020). Aktivitas Antimikroba Ekstrak Etil Asetat Jamur Laut Yang Diisolasi Dari Karang Lunak Sarcophyton Sp. Dari Perairan Desa Tumbak Kecamatan Pusomaen. Pharmacon, 9(1), 142. Https://Doi.Org/10.35799/Pha.9.2020.27421

Paiva, A. C. (2014). Natural deep eutectic solvents – Solvents for the 21st century. . ACS Sustainable Chemistry & Engineering,, 2(5), 1063–1071.

Putrajaya, F., Hasanah, N., & Kurlya, A. (2019). Daya Hambat Ekstrak Etanol Daun Suruhan (Peperomia Pellucida L.) Terhadap Pertumbuhan Bakteri Penyebab Jerawat (Propionibacterium Acnes) Dengan Metode Sumur Agar. Edu Masda Journal, 3(2), 123. Https://Doi.Org/10.52118/Edumasda.V3i2.34

Rahman, M. K., Fachriyah, E., & Kusrini, D. (2023). Ekstraksi Daun Salam Berbasis Natural Deep Eutectic Solvent Dan Pemanfaatannya Sebagai Antioksidan. Greensphere: Journal Of Environmental Chemistry, 2(2), 7–12. Https://Doi.Org/10.14710/Gjec.2022.16569

Santoso, U., Utari, M., & Marpaung, M. P. (2020). Aktivitas Antibakteri Dan Antijamur Ekstrak Batang Akar Kuning (Fibraurea Chloroleuca Miers) Terhadap Escherichia Coli, Staphylococcus Aureus dan Candida Albicans. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan, Analis Kesehatan Dan Farmasi, 20(2), 194. Https://Doi.Org/10.36465/Jkbth.V20i2.611

Ulfa, E. U., P, M. R. A. P., & Dianasari, D. (2024). Skrining Fitokimia Dan Evaluasi Antibakteri Ekstrak Nades ( Natural Deep Eutectic Solvents ) Daun Iler ( Coleus Atropurpureus L . Benth ) Terhadap Bakteri Penyebab Folikulitis. Journal Of Agropharmacy, 1(1), 1–5.

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Published

2025-06-01

How to Cite

Marliza, H., Utami, R. T., Amelia, A., & Pratiwi, R. (2025). Pengujian aktivitas antimikroba ekstrak daun kersen (Mutingia calabura L.) menggunakan pelarut nades (Natural Deep Eutectic Solvents) dengan metode disc diffusion. Arfak Chem: Chemistry Education Journal, 8(1), 737–747. https://doi.org/10.30862/accej.v8i1.889

Issue

Vol. 8 No. 1 (2025): Arfak Chem

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